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CompactDry CFR

Rapid coliforms in raw and pasteurized dairy products

Incubation Time
16 – 18 hours

Incubation Temperature
35 +/- 1°C (MicroVal)

Coliforms are gram-negative, facultative anaerobic, non-spore forming bacteria and commonly found in animal intestines and soil. The term "coliform" refers to enteric bacteria found in...

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Quantity

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40 plates 54070-CFR-0040
240 plates 54070-CFR-0240
1400 plates 54070-CFR-1400

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Coliforms are gram-negative, facultative anaerobic, non-spore forming bacteria and commonly found in animal intestines and soil. The term “coliform” refers to enteric bacteria found in food stuff such as meat, shellfish, dairy products, other foods and in particular water.

Bacteria such as Shigella, Salmonella, Yersinia, Citrobacter, Klebsiella, and Enterobacter are all coliforms, but the most recognized coliform is Escherichia coli. Testing for the presence of coliform is important in the food industry especially in monitoring water quality.

 

Advantages of using CompactDry CFR to test for coliforms in raw dairy products and heat processed dairy products.

Compact Dry CFR is a ready-to-use dry media sheets comprising culture medium and a cold-soluble gelling agent, rehydrated by inoculating 1 ml diluted sample into the centre of the selfdiffusible medium. This is a ready to use, chromogenic plate for the enumeration of Coliforms. Following incubation at 35±1°C for 16-18h Coliforms grow to give blue/blue-green colonies due to chromogens contained in the medium.

Monitoring and identification of coliforms is used at each step of the food production process as a quality control parameter. The CompactDry CFR selective media contains the chromogenic enzyme substrate X-Gal which result in a blue to blue/green colonies. CompactDry CFR enables easy culture and coliform count in dairy products and swab based environmental testing for surfaces and production areas.

The media contains the chromogenic enzyme substrate X-Gal and coliforms colonies are coloured blue/blue green.

Blue / Blue Green

General protocol

Step #1
1

Remove the cap

Step #2
2

Dispense 1 mL of sample in the middle of the CompactDry plate

Step #3
3

The sample diffuses passively and evenly across the dehydrated media sheet, rehydrating the dry medium into a gel within seconds

Step #4
4

Replace the lid and label the plate

Step #5
5

Turn over the plate (lid down) and incubate for the appropriate time and temperature

Step #6
6

Following incubation, count the number of coloured microbial colonies

Incubation Time
16 – 18 hours

Incubation Temperature
35 +/- 1°C (MicroVal)

Shelf Life
12 months

Storage Temperature
RT +1°C to +30°C

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